Jose Antonio Reyes during training session at National Stadium, Warsaw, Poland. File / Carl Recine / Reuters
Reyes shot to fame at Sevilla before a switch to Arsenal where he was part of the unbeaten 'Invincibles' 2003-2004 Premier League winners, before spells at Real and Atletico Madrid.
UEFA announced they would hold a minute's silence in his honour ahead of Saturday's Champions League final between Tottenham and Liverpool.
Former Arsenal boss Arsene Wenger signed Reyes on the back of Spain's Under-19's European championships win where he played alongside Fernando Torres and Andres Iniesta, and a dazzling series of matches with Sevilla the following season.
Arsenal described the accident as "awful news." "Reyes was, of course, a member of the Invincibles," Arsenal said on their website. "(He) made a significant contribution to that unbeaten season." "Thanks for what you gave us, and rest in peace," Arsenal said.
Jose Antonio Reyes celebrates after scoring the 4th goal for Arsenal against Middlesbrough. File / Reuters
He won Premier League and FA Cup medals at Arsenal and came on as a late substitute when the Gunners lost the 2006 Champions League final to Barcelona. But it was at Sevilla where the pain will be most keenly felt. "We couldn't be confirming worse news," Sevilla said when breaking the news.
"Beloved Sevilla star Jose Antonio Reyes has died in a traffic collision. Rest in peace," Sevilla, where he spent two four-year spells, said on Twitter.
He helped Sevilla win the Spanish second division in his first stint at the club. He won the Europa League five times, twice with Atletico Madrid and three times in his second spell at Sevilla.
The emergency services in Andalusia and the authorities in the town where his family live, Utrera, said Reyes died in an accident on the main road from Sevilla to Utrera. They said another member of his family also died and a third person was injured.
Spain captain Sergio Ramos, who is also from the province of Seville, came through the youth team just after Reyes and later played with him at Real Madrid also tweeted his grief.
"Broken. Shattered," wrote Ramos. "I have no words. All the affection for the family. We will always remember you, my friend! RIP brother."
Reyes played 21 times for Spain between 2003 and 2006, scoring four goals. He also won domestic league titles with Real Madrid and Benfica.
After leaving Sevilla in 2016, Reyes played for Espanyol, Cordoba and Xinjian Tianshan in China, before arriving at Extremadura this year as a free agent.
"With broken hearts we announce the news of our player Jose Antonio Reyes' death in a car crash and we ask for you to pray for his soul," Extremadura said.
Monchi, who overlapped with Reyes as a player at Sevilla and is now the club's director of football, also tweeted his sadness and referred to wingback Antonio Puerta, who died of a cardiac arrest playing for Sevilla in 2007.
"Impossible news to believe, impossible to digest, that hard my God," wrote Monchi. "What a left flank you are going to make, Antonio and you, up there
Barcelona might have their eighth title in 11 years all-but sewn up but, with six games left to play, La Liga is far from finished.
Barcelona midfielder Ivan Rakitic accepts his days at the Nou Camp may be numbered, but for now he is concentrating on preparing for next season with the Spanish champions.
Spain took a step closer to qualification for Euro 2020 on Thursday after surviving a scare in Romania to win 2-1 and continue their 100 per cent record, while Italy also kept their perfect
Indian spinners Axar Patel and Ravichandran Ashwin took seven wickets between them as England’s batting failed again on the first day of the fourth and final Test on Thursday.
Stefanos Tsitsipas had to dig deep to edge out Poland’s Hubert Hurkacz on Thursday in three sets to set up a quarter-final against Karen Khachanov in Rotterdam. The Greek second seed dropped the second set but won in a tight decider on his third
Organisers of the Dubai Duty Free (DDF) Tennis Championships have announced defending champion Simona Halep has withdrawn from the tournament. Halep, who was due to compete in Dubai for the eighth time, was looking forward to