The university will also ban smoking entirely on campus from August.
Nagasaki University spokesman Yusuke Takakura told AFP they have "stopped hiring any teaching staff who smoke", although applicants who promise to kick the habit before taking up their post could still be offered employment.
The university will also ban smoking entirely on campus from August, opening a clinic for those who cannot give up, said Takakura.
"We have reached a conclusion that smokers are not fit for the education sector," the spokesman said, adding that the university had sought legal advice and does not believe the policy contravenes discrimination laws.
Local media said it was the first state-run university to introduce such a condition of employment and the move comes after Tokyo's city government passed strict new anti-smoking rules last year ahead of the 2020 Summer Games.
Japan has long been an outlier in the developed world, considered a smoker's paradise where lighting up is allowed in many restaurants and bars.
Tokyo's new laws ban lighting up at restaurants in the capital, regardless of size. Restaurants can set up separate indoor smoking areas, but customers cannot eat or drink there.
Smoking is also banned entirely on school premises from kindergartens to high schools, although space can be set aside outside university and hospital buildings.
The World Health Organization has given Japan its lowest rating for efforts to prevent passive smoking, and it even scores poorly in the region compared with countries like China and South Korea.
Despite that, tobacco use in Japan has been falling in line with a broader global trend.
With skateboarding making its Olympic debut in Japan next year, Cuban skateboarders are hoping their sport will finally be accepted at home.
Yoshiro Mori announced his surprise resignation as honorary chairman of the Japan Rugby Football Union, officials said on Thursday, just months before the country hosts the World Cup.
Newly crowned Masters champion Tiger Woods announced on Thursday he will return to Japan for the first time in more than a decade to play in a new US PGA Tour event in October.
Protesters threw petrol bombs at the Tsim Sha Tsui police station on Kowloon peninsula after police inside fired volleys of tear gas to disperse demonstrators on the street.
Pakistan's army later said that "unprovoked cease-fire violations" by Indian troops killed five civilians and one soldier and wounded another three civilians and two troops across the highly militarized Line of Control that divides Kashmir between Pakistan and India.
Travellers to the UAE shall be receiving a three-shaded bookmark, marking the start of the year-long celebrations for the much-awaited Expo2020 Dubai scheduled for Oct.20, 2020 to April 10, 2021.